Recombinant DNA lab

The process for producing recombinant DNA is first to find a restriction enzyme that will cut the plasmid at one site and this same enzyme should be able to cut the cell DNA at two sites. Then we splice our insulin gene into the plasmid. Next we would add the enzyme ligase to glue the sticky ends together. We have now created a recombinant plasmdThere are different types of antibiotic resistances. Tetracycline, kanamycin, and ampiciliin are antibiotics which some plasmids provide resistance to it. I could use tetracycline and kanamycin to see if bacteria have taken in my plasmid because our plasmid doesn't have a resistance to those antibioics. I wouldn't use ampicillin because that is the antibiotic the plasmid is resistance to. Restriction enzymes are bacterial enzynes which are major tools of recombinant DNA techonology .We used Eco RI because it was the one closest to insulin and it cut the DNA twice. If I used an enzyme that cut the plasmid in two places, it wouldn't keep it's circular shape. This process is important in our everyday lives because it is essential to genetic engineering. It helps with diabetes by inserting the gene for insulin into a bacterial plasmid. This process can also be used to produce genetically modified food such as delaying fruit ripening and resistance to insects and plant viruses.